Integrated visualization of multi-angle bioluminescence imaging and micro CT
نویسندگان
چکیده
This paper explores new methods to visualize and fuse multi-2D bioluminescence imaging (BLI) data with structural imaging modalities such as micro CT and MR. A geometric, back-projection-based 3D reconstruction for superficial lesions from multi-2D BLI data is presented, enabling a coarse estimate of the 3D source envelopes from the multi-2D BLI data. Also, an intuitive 3D landmark selection is developed to enable fast BLI / CT registration. Three modes of fused BLI / CT visualization were developed: slice visualization, carousel visualization and 3D surface visualization. The added value of the fused visualization is demonstrated in three small-animal experiments, where the sensitivity of BLI to detect cell clusters is combined with anatomical detail from micro-CT imaging.
منابع مشابه
Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging
Background: Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c mice. Methods: The potential infectivity of recombinant L. tropicaEGFP or L. tropicaEGFP-LUC by two different, the subcutaneous and intradermal, routes was compared using a range of ...
متن کاملA Hybrid Method for Segmentation and Visualization of Teeth in Multi-Slice CT scan Images
Introduction: Various computer assisted medical procedures such as dental implant, orthodontic planning, face, jaw and cosmetic surgeries require automatic quantification and volumetric visualization of teeth. In this regard, segmentation is a major step. Material and Methods: In this paper, inspired by our previous experiences and considering the anatomical knowledge of teeth and jaws, we prop...
متن کاملComplementary Use of Bioluminescence Imaging and Contrast-Enhanced Micro-Computed Tomography in an Orthotopic Brain Tumor Model.
Small animal models are crucial to link molecular discoveries and implementation of clinically relevant therapeutics in oncology. Using these models requires noninvasive imaging techniques to monitor disease progression and therapy response. Micro-computed tomography (CT) is less studied for the in vivo monitoring of murine intracranial tumors and traditionally suffers from poor soft tissue con...
متن کامل3D Tumor Shape Reconstruction from 2D Bioluminescence Images and Registration with CT Images
This paper introduces a novel and efficient algorithm for reconstructing the 3D shapes of tumors from a set of 2D bioluminescence images which are taken by the same camera but after continually rotating the animal by a small angle. The method is efficient and robust enough to be used for analyzing the repeated imaging of a same animal transplanted with gene marked cells. There are several steps...
متن کاملMonte Carlo method for bioluminescence tomography.
Bioluminescence imaging plays an important role in the areas of cancer biology, cell biology, gene therapy, and so on. The 2D planar bioluminescent imaging has been transformed into a 3D framework by bioluminescence tomography (BLT) that enables bioluminescent source reconstruction in a mouse using a modality fusion approach. To solve this BLT problem, a geometrical model of the mouse is usuall...
متن کامل